Engineering alternative isobutanol production platforms

Engineering alternative butanol production platforms in heterologous bacteria.

Alternative microbial hosts have been engineered as biocatalysts for butanol biosynthesis. The butanol synthetic pathway of Clostridium acetobutylicum was first re-constructed in Escherichia coli to establish a baseline for comparison to other hosts. Whereas polycistronic expression of the pathway genes resulted in the production of 34 mg/L butanol, individual expression of pathway genes elevat...

Engineering Alternative Butanol Production Platforms in 1 Heterologous Bacteria

Metabolic engineering of Pichia pastoris for production of isobutanol and isobutyl acetate

Background Interests in renewable fuels have exploded in recent years as the serious effects of global climate change become apparent. Microbial production of high-energy fuels by economically efficient bioprocesses has emerged as an attractive alternative to the traditional production of transportation fuels. Here, we engineered Pichia pastoris, an industrial workhorse in heterologous enzyme p...

1 Metabolic Engineering of Clostridium Cellulolyticum for 1 Isobutanol Production from Cellulose 2 3 4

Metabolic engineering of Synechocystis sp. strain PCC 6803 for isobutanol production.

Global warming and decreasing fossil fuel reserves have prompted great interest in the synthesis of advanced biofuels from renewable resources. In an effort to address these concerns, we performed metabolic engineering of the cyanobacterium Synechocystis sp. strain PCC 6803 to develop a strain that can synthesize isobutanol under both autotrophic and mixotrophic conditions. With the expression ...